Isolation and Identification of Klebsiella pneumoniae using API-20E analytical system and conventional PCR assay
نویسندگان
چکیده
منابع مشابه
Isolation and identification of Campylobacter jejuni from poultry carcasses using conventional culture methods and multiplex PCR assay
Campylobacter jejuni is a major cause of food-borne diarrhea in many countries. Poultry and poultry products are known as important sources of human campylobacteriosis. In this study, conventional culture and multiplex PCR methods were compared for the detection of C. jejuni isolated from poultry carcasses. A total of 100 samples, representing 20 broiler flocks, were collected from poultry carc...
متن کاملRapid identification of Enterobacteriaceae with the micro-ID system versus API 20E and conventional media.
The Micro-ID system for rapid (4 h) identification of Enterobacteriaceae was evaluated by testing 433 enteric bacilli and 9 other gram-negative bacilli. Each isolate was identified with conventional tubed media and was also tested in the Micro-ID and API 20E systems. The overall accuracy of both systems was 97%. Micro-ID tests for the Voges-Proskauer reaction, indole and H2S production, and orn...
متن کاملIdentification of Yersinia spp. with the API 20E system.
The ability of the API 20E system to identify 105 clinical isolates of Yersinia spp. was compared with those of conventional biochemical tests at 28 and 37 degrees C. Elimination of the Voges-Proskauer test (recorded as a negative result) increased the percentage of correct identifications for Yersinia spp. from 66 to 93% when the API 20E strips were incubated at 28 degrees C.
متن کاملThe comparison of PCR technique and API-20E kit with the conventional biomedical methods for the identification of Salmonella species in laboratory
Abstract Bachground and objectives: Salmonella is one of the most important agents of gastrointestinal infection and diarrhea in our country. Misdiagnosis of these bacteria leads to cure failure. The aim of this study was to make a comparison between PCR and the API-20E and conventional biochemical tests carried out for the identification of Salmonella. Material and Methods: In this study 470 s...
متن کاملIdentification of Enterobacteriaceae by the API 20E system.
Since the introduction of the API 20E kit a number of identification schemes have been developed by the manufacturer for use with the kit. We evaluated the success of these various schemes in identifying 206 strains belonging to 34 taxa of the family Enterobacteriaceae. Many of the strains were atypical and only 94% could be identified by our own system of 50 conventional tests and a computer p...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: International Journal of Current Microbiology and Applied Sciences
سال: 2017
ISSN: 2319-7692,2319-7706
DOI: 10.20546/ijcmas.2017.608.028